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. 2023 Aug 8;14:1225586. doi: 10.3389/fphar.2023.1225586

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Copyright © 2023 Molonia, Salamone, Muscarà, Costa, Vento, Saija, Speciale and Cimino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C3G effect on Mitotic Clonal Expansion in 3T3-L1 cells treated in early phase. (A) Effect on cell number: cell count was determined after trypsinization on day 4 by hemocytometer counts. (B–C) Effect on cell cycle arrest. (D) Effect on p21 protein levels: (D1) Representative images from four independent experiments. (D2) Densitometry data are reported as fold change vs. MDI control. The order of histogram bars was rearranged for illustrative purposes. All data are expressed as mean ± SD of four independent experiments (n = 4) each in triplicate. Means with the same letter are not significantly different from each other (p > 0.05).