TABLE 4.
Genes that were differentially regulated between oocytes prior the final injection and stripped eggs with FC (P): fold change resulting from the comparison between oocytes sampled prior to P injection and eggs stripped after P injection; FC (DHP): fold change resulting from the comparison between oocytes prior the DHP injection and eggs stripped after DHP injection. The * represents the leveling.
| *Apoptosis | FC (P) | FC (DHP) | *Lipid metabolism | FC (P) | FC (DHP) |
|---|---|---|---|---|---|
| DNA damage-inducible transcript 4-like protein | 61 | 15 | phospholipid-transporting ATPase ABCA1 | −39 | −26 |
| serine/threonine-protein kinase 17A-like | −31 | −8 | lipoprotein lipase | −514 | −143 |
| *Cell adhesion | alpha-2,8-sialyltransferase 8F-like | inf | −119 | ||
| catenin (cadherin-associated protein), delta 1 | 24 | 13 | protein ABHD15-like | inf | −234 |
| carcinoembryonic antigen-related cell adhesion molecule 5 | −282 | −152 | *Muscle contraction | ||
| cadherin-2-like | inf | −53 | tropomodulin-3-like | 26 | 11 |
| *Cell cycle | myosin-7-like | inf | inf | ||
| cell division cycle 25B | 74 | 48 | *Steroid signalling pathway | ||
| geminin DNA replication inhibitor | 70 | 32 | CUE domain containing 2 | 27 | 41 |
| anillin, actin binding protein | 45 | 27 | progesterone receptor | −327 | −246 |
| cyclin A1 | 19 | 12 | estrogen receptor 2b | −15 | −39 |
| cyclin-J-like | 18 | 17 | progesterone receptor-like | inf | −74 |
| cyclin I | −55 | −19 | androgen receptor-like | inf | −33 |
| *Ion, water, protein transport | *Steroidogenesis | ||||
| sorting nexin-13-like | 57 | 16 | steroid 17-alpha-hydroxylase/17,20 lyase, transcript 1 | −5040 | −4167 |
| protein Hook homolog 2-like | 25 | 27 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase-like | −135 | −42 |
| vesicle-fusing ATPase-like | 24 | 27 | hydroxysteroid (17-beta) dehydrogenase 1 | inf | −258 |
| sorting nexin-13-like | 57 | 16 | steroid 17-alpha-hydroxylase/17,20 lyase, transcript 2 | inf | −36 |
| SIL1 nucleotide exchange factor | −114 | −51 | *Cytoskeleton organization | ||
| ATPase Na+/K+ transporting subunit beta 1a | −153 | −89 | tubulin beta chain-like | −73 | −14 |
| ammonium transporter Rh type A-like | −565 | −123 | tubulin alpha 5 | −48 | −9 |
| 4F2 cell-surface antigen heavy chain-like | −35 | −9 | tubulin alpha chain-like | −96 | −32 |
| claudin-19-like | inf | −145 | tubulin beta chain-like | −21 | −12 |
| putative claudin-24 | inf | −101 | *Extracellular matrix organization | ||
| potassium inwardly rectifying channel subfamily J member 8 | inf | inf | angiopoietin-like 7 | inf | inf |
| aquaporin-4-like | inf | −508 | A disintegrin and metalloproteinase with thrombospondin motifs 5 | −3232 | −44 |
| collagen alpha-1 (XV) chain-like | −203 | −84 |
Inf for infinite: expression in the eggs was 0. Between the injection moment and egg release, several genes were involved in apoptosis, cell adhesion, cell cycle, cytoskeleton organization, extracellular matrix organization, transport, lipid metabolism, muscle contraction, steroid signalling pathway and steroidogenesis.