Table 1.
Genotypes and sources of the S. cerevisiae strains used in this study.
| Strain | Relevant genotype | mt DNA | Source |
|---|---|---|---|
| W303-1A | MATa leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 | ρ+ | R Rothstein, Columbia University |
| W303-1B | MATα leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 | ρ+ | R Rothstein, Columbia University |
| MR6 | MATa ade2-1 his3-11,15 trp1-1 leu2-3,112 ura3-1 Δarg8::HIS3 | ρ+ | Rak et al (2007) |
| MRS-3A | MATa leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 Δarg8::HIS3 | ρ+ | McStay et al (2013) |
| MRS-3B | MATα leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 Δarg8::HIS3 | ρ+ | McStay et al (2013) |
| aM9-94/4B | MATa ade1 | cox2 | Coruzzi and Tzagoloff (1979) |
| M9-94/A1 | MATα met | cox2 | Coruzzi and Tzagoloff (1980) |
| MRS/COX3-HAC | MATα leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 Δarg8::HIS3 | ρ+ COX3-HACa | Su et al (2014b) |
| MRS/COX2-pH | MATα leu2-3,112 trp1-1 ura3-1 ade2-1 his3-11,15 Δarg8::HIS3 | ρ+ COX2-pHb | Franco et al (2018) |
| DFKρ0 | MATα kar1-1 ade2-101 leu2D ura3-52 lys2 Δarg8::URA3 | ρ0 | McStay et al (2013) |
| DFK/VAR1/ST12ρ- | MATα kar1-1 ade2-101 leu2D ura3-52 lys2 Δarg8::URA3 | ρ- 3′VAR1::ARG8m | This studyc |
| aMRS/VAR1/ST12ρ+ = aMRS-A9R | MATa ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δarg8::HIS3 | ρ+ 3′VAR1::ARG8m | This studyc |
| aMRS/VAR1/ST13ρ+ = aMRS-sA9R | MATa ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δarg8::HIS3 | ρ+ 3′VAR1::ARG8m | This studyd |
| DFK/VAR1/ST9ρ- | MATα kar1-1 ade2-101 leu2D ura3-52 lys2 Δarg8::URA3 | ρ- 3′VAR1::COX6-Cm | This studye |
| aW303ΔCOX6 | MATa ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1Δ cox6::URA3 | ρ+ | Glerum and Tzagoloff (1997) |
| aW | MATa ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δcox6::URA3 | ρ0 | This study |
| a/αW303 ΔCOX6/COX6-Cm | MATa,α ade2-1/ade2-1 his3-1,15/his3-1,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1 Δcox6::URA3/Δcox6::URA3 | ρ+ 3′VAR1:: COX6-Cm | This study |
| aW303 ΔCOX6/COX6-Cm | MATa ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δcox6::URA3 | ρ+ 3′VAR1:: COX6-Cm | This study |
| W303/COX6-HAC | MATα ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δcox6::URA3 trp1::pG71/ST9f | ρ+ | McStay et al (2013) |
| W303/COX6-C | MATα ade2-1 his3-1,15 leu2-3,112 trp1-1 ura3-1 Δcox6::URA3 trp1::YIp349-COX6-3met-C#7g | ρ+ | This study |
| aMRSΔATP9/COX6-HAC | MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 Δcox6::URA3 trp1::pG71/ST9f Δarg8::HIS3 | ρ+ Δatp9:: ARG8m | This study |
a
COX3 fused at the 3′ end to a sequence coding for hemagglutinin followed by the protein C tag.
b
COX2 fused at the 3′ end to a sequence-coding for seven histidines.
c
(See Fig 1A).
d
(See Fig 1B).
e
(See Fig 3A).
f
pG71/ST9 consists of the integrative TRP1 plasmid YIp349 with COX6 fused at its 3′ end to a sequence-coding for the hemagglutinin followed by the protein C tag.
g
YIp349-COX6-3met-C#7 consists of the integrative TRP1 plasmid YIp349 with COX6 fused at its 3′ end to a sequence coding for three methionine codons followed by the protein C tag.