Table 2.
Additional E2 conjugating enzymes screened for their ability to be loaded with FAT10.
| E2 conjugating enzyme | Alternative name | In vitro loading with FAT10 | In cellulo loading with FAT10 | Reconstitution of FAT10 conjugation in USE1-ko cells |
|---|---|---|---|---|
| UBE2A | RAD6A | yes | yes | yes |
| UBE2B | RAD6B | yes | yes | yes |
| UBE2C | UbcH10 | yes | yes | no |
| UBE2D1 | UbcH5A | yes | yes | yes |
| UBE2D2 | UBcH5B | yes | n.d. | not expressed |
| UBE2D3 | UBcH5C | yes | yes | yes |
| UBE2D4 | UBcH5D | no | n.d. | n.d. |
| UBE2E1 | UBCH6 | yes | n.d. | not expressed |
| UBE2E2 | UBCH8 | no | n.d. | n.d. |
| UBE2E3 | UBCH9 | no | n.d. | n.d. |
| UBE2G1 | UBC7 | no | n.d. | n.d. |
| UBE2G2 | UBC7 | yes | yes | yes |
| UBE2T | FANCT | no | n.d. | n.d. |
n.d., not determined; not expressed: no protein expression was detectable by Western blot analysis.