FIG. 2.

MV-Ed-infected T cells upregulate but do not downregulate CD69 after stimulation compared to mock-infected T cells. T cells were labeled with CFSE, mock (bold curve) or MV-Ed (fine curve) infected, and left unstimulated (D) or cultured with PHA (2 μg/ml) and IL-2 (50 U/ml) (A to C). Cells were surface labeled with antibodies specific to CD69 followed by a secondary phycoerythrin-conjugated immunoglobulin and then analyzed by flow cytometry at 24 h, 72 h, and 4 days p.i. Panel D shows CD69 expression on nonstimulated T cells at 72 h p.i.