FIG. 6.

Upregulation of Rb protein is impaired in MV-infected stimulated T cells. T cells were mock or MV-Ed infected, cultured with PHA and IL-2, and lysed at equal numbers in SDS-PAGE sample buffer at 4 days p.i. Lysates were subjected to SDS-PAGE, proteins were transferred onto PVDF filters, and blots were probed with anti-Rb clone G3-245 (Pharmingen). Lanes 1 to 3 show nonstimulated cells, stimulated mock-infected cells, and stimulated MV-Ed-infected cells, respectively. The number at the left is a molecular mass, in kilodaltons.