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. 2023 Jul 31;12(8):2432–2443. doi: 10.1021/acssynbio.3c00295

Figure 3.

Figure 3

Introduction of other SYNZIP pairs into XtpS. Two parallel interacting SYNZIPs, SZ2:19 and SZ4:21, were introduced into the A–T position of module two to generate NRPS-14 and NRPS-15. Corresponding peptide yields (milligrams per liter) and standard deviations are obtained from biological triplicate experiments. Domain assignment is as described before. For domain assignment, the following symbols are used: (A, large circles), (T, rectangle), (C, triangle), (C/E, diamond), (TE, small circle); substrate specificities are assigned for all A-domains and indicated by capital letters.