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. 2023 Jul 31;12(8):2432–2443. doi: 10.1021/acssynbio.3c00295

Figure 6.

Figure 6

Introduction of a ring network. (A) Applied orthogonal and ring networks. SZ17:18 and SZ1:2 form an orthogonal network, meaning that both SYNZIPs do not interact with each other. Introducing two SZ17:18 pairs results in a ring network whereby applied SYNZIPs cross-talk. (B) For the construction of a ring interaction network, SZ1:2 in NRPS-47 was changed against SZ17:18, resulting in NRPS-48. NRPS-48 was capable to incorporate subunit B not at all or up to three times (NRPS-18a to -18c), leading to the production of peptides 15–18 shown at the bottom. Corresponding peptide yields (mg/L) and standard deviations are obtained from biological triplicate experiments. For domain assignment, the following symbols are used: (A, large circles), (T, rectangle), (C, triangle), (C/E, diamond), (TE, small circle); substrate specificities are assigned for all A-domains and indicated by capital letters.