Table 1. CRISPRi/Overexpression Gene Targets.
| target gene | category | function | essential(E. coli)a | essential (φT7)b | ref. |
|---|---|---|---|---|---|
| infC | protein | translation initiation factor IF-3 | yes | (32) | |
| pgk | energy | phosphoglycerate kinase | yes | (32) | |
| subH (ssyA) | energy | inositol monophosphatase | yes | (32) | |
| hemL (gsa) | protein | glutamate-1-semialdehyde aminotransferase | yes | (32) | |
| eno | energy/nucleic acid | enolase in glycolysis/gluconeogenesis/mRNA degradosome | yes | (32) | |
| mukB | protein/cell-division | degradosome/chromosomal DNA condensation | yes | (32) | |
| lexA | transcription factor | SOS response regulator | yes | (32) | |
| rne | nucleic acid | RNase E | yes | no | (29,32,33) |
| nusG | transcription factor | transcription termination factor | yes | no | (32,34,35) |
| trxA | redox regulator | oxidized thioredoxin | no | yes | (29,32) |
| T7gp3 (T7 gene) | nucleic acid | φT7 DNA replication | no | yes | (36) |
| dgt | energy | deoxyguanosinetriphosphate (dGTP) triphosphohydrolase | no | no | (29,32) |
| udk | nucleic acid | uridine kinase/cytidine kinase | no | no | (29,32) |
| recC | nucleic acid | Exodeoxyribonuclease V (RecBCD) subunit | no | no | (29,32,37) |
| cyaR (ryeE) | transcription factor | CyaR small RNA, oxidative stress regulator | no | (38) | |
| rna | nucleic acid | Rnase I, cleaves phosphodiester bond between any two nucleotides | no | (32) | |
| oxyS | transcription factor | OxyS small RNA, oxidative stress regulator | no | no | (39) |
| trxB | redox regulator | thioredoxin reductase | no | no | (29,32,40) |
a
Chromosomal deletion results in nonviable or nonreplicative cells.
b
Host/phage chromosomal deletion causes nonproductive phage infections.