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. 2023 Jun 16;16(9):1755–1773. doi: 10.1111/1751-7915.14297

FIGURE 2.

FIGURE 2

Peptide MSI‐1 decreased protein constituents, especially many established virulent proteins, enriched in OMVs of BL21/pMCR‐1. (A) SDS‐PAGE results of protein profiles in OMVs from BL21/pEmpty, BL21/pMCR‐1 and sBL21/pMCR‐1 strains. (B, C) Samples were separated by 12% SDS‐PAGE and visualized by Coomassie blue staining. The content of key virulent proteins (B) and MCR‐1 (C) in BL21/pEmpty‐OMVs, BL21/pMCR‐1‐OMVs and sBL21/pMCR‐1‐OMVs by label‐free quantitative proteomics. (D) Venn graph of differentially expressed proteins (DEPs) overlapping between BL21/pEmpty‐OMVs and BL21/pMCR‐1‐OMVs (D, left) and the numbers of upregulated and downregulated DEPs from BL21/pMCR‐1‐OMVs compared with BL21/pEmpty‐OMVs (D, right). (E) The DEPs between BL21/pEmpty‐OMVs and BL21/pMCR‐1‐OMVs were categorized based on the related biological process, cellular component and molecular function. (F) Venn graph of DEPs overlapping between BL21/pMCR‐1‐OMVs and sBL21/pMCR‐1‐OMVs (F, left) and the numbers of upregulated (red) and downregulated (green) DEPs from BL21/pMCR‐1‐OMVs and BL21/pMCR‐1‐OMVs compared with sBL21/pMCR‐1‐OMVs (F, right). (G) The DEPs between BL21/pMCR‐1‐OMVs and sBL21/pMCR‐1‐OMVs were categorized based on the gene ontology terms biological process, cellular component and molecular function.