FIGURE 2.

Structure of PlyGRCS. (a) A representative 2m|Fo| ‐ D|Fc| map contoured at 1.0 ϭ level corresponding to the linker region (shown in cyan sticks). A long flexible region (N141 ‐ N154) connects the CHAP_GRCS (yellow) and SH3_5_GRCS (green) domains. The map is shown as brown mesh. (b) Near the Ca²⁺ binding region. The Ca²⁺ ion is coordinated with Asp20, Asp22, Ala24, Gly26, and Asp31. (c) Near the catalytic site region. Cys29, His89, and Glu105, the conserved catalytic residues, are shown as sticks. (d) Near the interface between CHAP_GRCS and SH3_5_GRCS. The residues involved in the intermolecular interactions are shown as yellow and green sticks, respectively, corresponding to the CHAP_GRCS and SH3_5_GRCS regions.