Table 1. Predicted Number of gRNA That Could be Used for Genetic and Genetic Interaction Screens^a.

			# of good probes		# of gRNA
experimental setup	#laser	noise	one barcode	two barcodes	genetic screen	genetic interaction screen
Cytek Northern Lights	3	high	337 ± 3	113 ± 5	56 737 ± 1001	1565 ± 146
	3	low	634 ± 5	230 ± 11	200 897 ± 3149	6544 ± 610
	2	high	292 ± 8	92 ± 5	42 458 ± 2445	1024 ± 101
	2	low	550 ± 25	175 ± 12	151 970 ± 14 008	3805 ± 528
Cytek Aurora	5	high	666 ± 15	294 ± 7	221 893 ± 10 020	10 694 ± 510
	5	low	894 ± 3	708 ± 12	399 477 ± 2596	62 397 ± 2046
	4	high	580 ± 9	252 ± 10	167 983 ± 4916	7860 ± 654
	4	low	879 ± 2	590 ± 11	385 885 ± 1755	43 294 ± 1535

^aResults for the number of good probes that can be used to form barcodes and pairs of barcodes are shown for each experimental setup (the flow cytometer used), the number of lasers used, and the noise level (either low or high). Given the number of good probes, the number of potential gRNA for genetic and genetic interaction screens is calculated, as shown in Figure 1E. Results for the Cytek Northern Lights flow cytometer are highlighted in blue, and the results for the Aurora flow cytometer are highlighted in yellow. Uncertainty is the standard deviation from triplicate simulation experiments.