Table 1.
Oligonucleotide sequences.
| SNP | Name (concentration, µM) |
5’–3’ sequence |
|---|---|---|
| rs138446575 | 6575-F (0.4) | CACCTGGCAACTTGCAGACAG |
| 6575-R (0.4) | biotin-AGGACGTCCTCGCCACAT | |
| 6575-S (0.3) | GGTGCCGCCAGCCTT | |
| 6575-C (0.04) | (FAM)G+CC+TT+CCTG+GGT (BHQ1) | |
| 6575-T (0.12) | (R6G)AG+CC+TT+TC+TGG+GT(BHQ1) | |
| rs55986091 | 6091-F (0.6) | TCTTTTTTATTTTTCCTAAGAGTCGAT |
| 6091-R (0.6) | biotin-GCAATTTTAATTTCGCCTCAGTC | |
| 6091-S (0.3) | GCATCTCATACACCACCATGCAC | |
| 6091-S2 (0.6) | CAAAAACCAGCAAGTTTTTATTAG | |
| 6091-G (0.12) | (FAM)T+TA+TT+AG+GGA+T+TT+TCA (BHQ1) | |
| 6091-A (0.12) | (R6G)T+T+AT+TAG+AG+A+TT+T+TCAA (BHQ1) | |
| rs1048943 | 8943-F (0.4) | CGACAAGGTGTTAAGTGAGAAGGTG |
| 8943-R (0.4) | biotin-AGGATAGCCAGGAAGAGAAAGACC | |
| 8943-S (0.3) | GGAAGTGTATCGGTGAGACC | |
| 8943-A (0.08) | (FAM)GA+GACC+AT+TGC+CC (BHQ1) | |
| 8943-G (0.12) | (R6G)G+AG+ACC+G+TT+GCC (BHQ1) | |
| rs2910164 | 10164-F (0.4) | CTGAATTCCATGGGTTGTGTCAGT |
| 10164-R (0.4) | biotin-GATGACAGAGATATCCCAGCTGAA | |
| 10164-S (0.3) | GGTTGTGTCAGTGTCAGACCT | |
| 10164-G (0.04) | (FAM)C+AG+ACCT+GTG+A+AA+TT(BHQ1) | |
| 10164-C (0.12) | (R6G)C+AG+ACCT+CTG+A+AA+TT(BHQ1) |
FAM, R6G – fluorophores; BHQ1 – a quencher. The LNA (locked nucleic acid) is indicated as “+_”. F, a forward primer; R, a reverse primer; S, a primer for pyrosequencing; S2, a primer for Sanger sequencing. The polymorphic nucleotides are highlighted in bold.