FIG. 4.

The AP-binding dileucine-based motif of Nef is dispensable for MHC-I down-regulation in HIV-1 infected cells. (A) Surface-level analysis of MHC-I in CEM-GFP cells infected by virions produced from 293T cells. GFP-positive cells were selected and analyzed for cell surface expression of MHC-I with a monoclonal antibody against the MHC-I heavy chain. (B) Immunoblot analysis of viral particles produced from 293T cells transfected with wild-type virus (R9) or the indicated nef-mutated variants, using antibodies against Nef (top) or p24 CA (bottom).