FIG. 5.

Interactions of Nef mutants with the endocytic apparatus. (A) Internalization rates of chimeras composed of the extracellular and transmembrane domains of CD4 and Nef as the cytoplasmic domain in 293T fibroblasts. Graphs are representative of two independent experiments. To combine the results of different experiments, results were normalized to the 44Nef and 44MA controls. (B) Diagram of the interaction measured in this cis-endocytosis assay. The chimeric integral membrane protein with Nef as the cytoplasmic domain is internalized at a rate that reflects its association the cell surface AP-2, a component of CCP.