Figure 6. L. major intracellular proliferation rate modifies host cell metabolic pathways.

(A–D) Gating and normalized fluorescence for 2-NBDG uptake (A and B) and CD36 (C and D) in uninfected (gray), Lm^DsRed proliferation-competent–infected (green), and Lm^DsRed KBMA–infected (red) BMDMs. Each symbol shows 1 individual biological replicate. Horizontal bars denote the mean. Data pooled from 3 independent experiments. *P < 0.05, **P < 0.01 by 1-way ANOVA with Bonferroni’s posttest. (E) Gating for CD11c⁺Ly6C^hi and CD11c⁺Ly6C^lo CD45.1⁺ monocytes (top panel) and histogram plots showing proliferation index in infected CD11c⁺Ly6C^hi (green) and CD11c⁺Ly6C^lo (red) (bottom panel) CD45.1⁺ cells. (F) Quantification of proliferation index normalized to the total proliferation index in each sample (see Methods) in infected CD11c⁺Ly6C^hi (green) and CD11c⁺Ly6C^lo (red) monocytes in newly recruited CD45.1⁺ cells analyzed according to gating shown in E. Each symbol represents 1 mouse ear. Horizontal bars denote the mean. Data pooled from 2 independent experiments. ***P < 0.001 by paired t test. (G) Gating for CD11c⁺Ly6C^hi and CD11c⁺Ly6C^lo monocytes (top panel) and histogram plots showing CD36 expression in infected and uninfected CD11c⁺Ly6C^hi (green) and CD11c⁺Ly6C^lo (red) (bottom panel) CD45⁺ cells. (H) MFI for CD36 expression in infected (squares) and uninfected (circles) CD11c⁺Ly6C^hi (green) and CD11c⁺Ly6C^lo (red) CD45⁺ monocytes analyzed according to gating shown in G. Each symbol represents 1 mouse ear. Horizontal bars denote the mean. Data pooled from 2 independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001 by 1-way ANOVA with Bonferroni’s posttest. (I–L) Gating and normalized LDL (I and J) and long-chain fatty acid (C16) (K and L) uptake in uninfected (gray), Lm^DsRed proliferation-competent–infected (green), and Lm^DsRed KBMA–infected (red) BMDMs. Each symbol shows 1 individual sample. Data pooled from 3 independent experiments. Horizontal bars denote the mean. *P < 0.05, **P < 0.01, ****P < 0.0001 by 1-way ANOVA with Bonferroni’s posttest.