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. 2023 Jul 24;8(14):e156529. doi: 10.1172/jci.insight.156529

Figure 5. Dose-dependent radiation damage in mouse tissues correlates with the origins of methylated cfDNA in the circulation.

Figure 5

(A) Representative H&E staining of lung, heart, and liver tissues from mice treated with 3 Gy or 8 Gy radiation compared to sham control. Scale bar: 200 μm. (B) qPCR analysis of Cdkn1a (p21) mRNA. The expression in each sample was normalized to Actb and is shown relative to the expression in the sham control. Mean ± SD; n = 3. Kruskal-Wallis test was used for comparisons among groups: lung tissue, P = 0.004; heart tissue, P = 0.025; liver tissue, P = 0.004. (C) Lung endothelial, cardiomyocyte, and hepatocyte methylated cfDNA in the circulation of mice treated with 3 Gy and 8 Gy radiation compared to sham control expressed in genome equivalents per mL serum (Geq/mL). cfDNA was extracted from 18 mice (n = 6 in each group), with cfDNA from 2 mice pooled in each methylome preparation. Mean ± SD; n = 3 independent methylome preparations. Kruskal-Wallis test was used for comparisons among groups. NS, P ≥ 0.05; *P < 0.05: lung endothelial, P = 0.01; cardiomyocyte, P = 0.01; hepatocyte, P = 0.13.