FIG. 1.

Mapping of the epitope recognized by MAb 1424 with fragments derived from the NS1 protein. Fragments of the NS1 protein were produced in E. coli, purified, and tested in an ELISA for the ability to bind MAb 1424. (A) Schematic representation of overlapping protein fragments NS/A to NS/D. Fragments recognized by the NS1-specific MAb are in black. (B) Reactivity of MAb 1424 with different NS1 subfragments tested by ELISA. Only fragment NS/D was strongly recognized by MAb 1424. OD₄₀₅, optical density at 405 nm.