FIG. 3.

Mapping of the epitope recognized by MAbs 1418-1 and 1418-16 by using VP1 protein fragments. Fragments of the VP1 protein were produced in E. coli, bound on ELISA plates precoated with antibodies against a polyhistidine stretch without prior purification, and tested in an ELISA for the ability to bind MAbs 1418-1 and 1418-16. (A) Schematic representation of overlapping protein fragments VP/A to VP/C. Fragments recognized by the VP1-specific MAbs are in black. (B) Reactivity of MAbs 1418-1 (grey columns) and 1418-16 (black columns) with different VP1 subfragments tested by ELISA. Only fragment VP/A was strongly recognized by both MAbs. OD₄₀₅, optical density at 405 nm.