FIG. 4.

The BaLCV and RhLCV Qp promoters are negatively autoregulated. (Left panel) EBV-negative Louckes BL cells were cotransfected with a BaLCV or RhLCV Qp reporter plasmid and either an empty expression vector (pSG5) or an EBNA-1 expression vector (pSG.E1) encoding BaLCV or RhLCV EBNA-1, respectively. (Right panel) BaLCV and RhLCV Qp reporter plasmids lacking a functional EBNA-1 binding domain (QpΔ34) are unresponsive to the respective LCV EBNA-1. Data are from a representative experiment in which all transfections were done in triplicate and hGH values were corrected for transfection efficiency. The reporter plasmids used in these experiments had the same 5′ and 3′ coordinates as indicated for Fig. 3. Error bars indicate standard deviations.