Table 3.

Primary and secondary IgG antibodies used for the immunodetection of RITs.

Antibody	Abbreviation	Conjugate	Manufacturer	Dilution (v v−1)	Conc. [g L−1]	RRID1
Goat-anti-mCherry	n.a.	none	Invitrogen	1:5000	1.0	AB_2552323 (PA5–34974)
Mouse-anti-VisA	TA-5	none	Fraunhofer IME	1:5000	0.8	n.a.
Rabbit-anti-His6	n.a.	none	GenScript	1:5000	0.8	AB_914703 (A00174)2
Rabbit-anti-ETA′	n.a.	none	Sigma-Aldrich	1:10,000	n.a.	AB_260908 (P2318)
Rabbit-anti-goat	RAG	AP	Invitrogen	1:5000	0.7	AB_228392 (31300)
Goat-anti-mouse	GAM	AP	Jackson ImmunoResearch	1:5000	0.8	AB_2338528 (115-055-003)
Goat-anti-rabbit	GAR	AP	Jackson ImmunoResearch	1:5000	0.8	AB_2337947 (111-055-003)
Goat-anti-human	GAH	AP	Merck	1:5000	1.2	n.a. (AP113A-K)

AP – alkaline phosphatase; ETA′ – Pseudomonas aeruginosa exotoxin; His₆ – Hexa-histidine; n.a. – not applicable; ¹ in case an RRID was not available, only a catalog number is given in brackets; ² the RRID description conflicts with the correct description on the manufacturer’s website, i.e. the antibody is in fact unconjugated.