Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | OP50-1 | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00041971 | Standard laboratory stock |
| Strain, strain background (Escherichia coli) | HT115(DE3) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00041079 | Background strain for RNAi clones utilized from Ahringer and Vidal Libraries |
| Strain, strain background (Caenorhabditis elegans) | Bristol N2 (wt) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00000001 | Standard laboratory wild-type strain |
| Strain, strain background (Caenorhabditis elegans) | fard-1(wa28) [G261D] | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00004025 | BX275 |
| Strain, strain background (Caenorhabditis elegans) | acl-7(wa20) [R234C] | Caenorhabditis Genetics Center | RRID: WS-STRAIN:WBStrain00004024 | BX259 |
| Strain, strain background (Caenorhabditis elegans) | ads-1(wa3) [G454D] | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00004007 | BX10 |
| Strain, strain background (Caenorhabditis elegans) | daf-2(e1370) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00004309 | CB1370 |
| Strain, strain background (Caenorhabditis elegans) | isp-1(qm150) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00026672 | MQ989 |
| Strain, strain background (Caenorhabditis elegans) | raga-1(ok701) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00035849 | VC533 |
| Strain, strain background (Caenorhabditis elegans) | eat-2(da465) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00005463 | DA465 |
| Strain, strain background (Caenorhabditis elegans) | mgIs43[ges-1p::GFP::PTS1] | Soukas Laboratory | N/A | MGH48 |
| Strain, strain background (Caenorhabditis elegans) | skn-1(lax188) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00034420 |
skn-1gf, SPC168 |
| Strain, strain background (Caenorhabditis elegans) | agIs6[dod-24p::GFP] | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00004921 | CF3556 |
| Strain, strain background (Caenorhabditis elegans) | dvIs19[(pAF15)gst-4p::GFP::NLS] | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00005102 | CL2166 |
| Strain, strain background (Caenorhabditis elegans) | skn-1(zu135) | Caenorhabditis Genetics Center | RRID: WB-STRAIN:WBStrain00007251 | skn-1lf, EU31 |
| Genetic reagent (Caenorhabditis elegans) | alxEx122[fard-1p::FARD-1::mRFP::HA unc-54 3'UTR myo-2p::GFP] | This study | MGH471 | fard-1 (oe1) |
| Genetic reagent (Caenorhabditis elegans) | alxEx135[fard-1p::FARD-1::mRFP::HA unc-54 3'UTR myo-2p::GFP] | This study | MGH472 | fard-1 (oe2) |
| Genetic reagent (Caenorhabditis elegans) | alxIs45[fard-1p::FARD-1::mRFP::HA::unc-54 3'UTR myo-2p::GFP] | This study | MGH605 |
fard-1 (oe3), backcrossed into N2 8× |
| Genetic reagent (Caenorhabditis elegans) | alxIs46[fard-1p::FARD-1::mRFP::HA::unc-54 3’UTR myo-2p::GFP] | This study | MGH606 | fard-1 (oe4), backcrossed into N2 8× |
| Genetic reagent (Caenorhabditis elegans) | mgIs43[ges-1p::GFP::PTS1]; alxEx122[fard-1p::FARD-1::mRFP::HA::unc-54 3’UTR myo-2p::GFP] | This study | MGH607 | GFP::PTS1; FARD-1::RFP, prepared by crossing MGH48 into MGH471 |
| Sequence-based reagent | 5’-TGCATGCCTGCAGGTCGACTTTGACAAAAGTTCTGTTGCCG-3’ | This study | AS-4524 | Forward primer used to generate fard-1 overexpression construct |
| Sequence-based reagent | 5’-TTTGGGTCCTTTGGCCAATCGCTTTTTTGAAGATACCGAGAATAATCC-3’ | This study | AS-4527 | Reverse primer used to generate fard-1 overexpression construct |
| Sequence-based reagent | 5’-TGCTGATCGTATGCAGAAGG-3’ | This study | act-1 F | qRT-PCR Primer |
| Sequence-based reagent | 5’-TAGATCCTCCGATCCAGACG-3’ | This study | act-1 R | qRT-PCR Primer |
| Sequence-based reagent | 5’-GTTCCCGTGTTCATCACTCAT-3’ | This study | pmp-3 F | qRT-PCR Primer |
| Sequence-based reagent | 5’-ACACCGTCGAGAAGCTGTAGA-3’ | This study | pmp-3 R | qRT-PCR Primer |
| Sequence-based reagent | 5’-ACAAGTCACCAATGGCTCCAC-3’ | This study | fard-1 F | qRT-PCR Primer |
| Sequence-based reagent | 5’-GCTTTGGTCAGAGTGTAGGTG-3’ | This study | fard-1 R | qRT-PCR Primer |
| Sequence-based reagent | 5’-cgatagtgtgtctgttgattgtga-3’ | This study | fard-1 F (Native 3’ UTR) | qRT-PCR Primer |
| Sequence-based reagent | 5’-agttattgttgatgagagagtgcg-3’ | This study | fard-1 R (Native 3’ UTR) | qRT-PCR Primer |
| Sequence-based reagent | 5’-GTTTATGGCTGGCGTGTTG-3’ | This study | acl-7 F | qRT-PCR Primer |
| Sequence-based reagent | 5’-CGGAGAAGACAGCCCAGTAG-3’ | This study | acl-7 R | qRT-PCR Primer |
| Sequence-based reagent | 5’-GCGATTAACAAGGACGGACA-3’ | This study | ads-1 F | qRT-PCR Primer |
| Sequence-based reagent | 5’-CGATGCCCAAGTAGTTCTCG-3’ | This study | ads-1 R | qRT-PCR Primer |
| Chemical compound, drug | C1-BODIPY-C12 (green) | Invitrogen | Cat#D-3823 | N/A |
| Chemical compound, drug | 5-fluoro-2′-deoxyuridine (FUdR) | Fisher Scientific | Cat#F10705 | N/A |
| Chemical compound, drug | Metformin hydrochloride | MilliporeSigma | Cat#PHR1084 | N/A |
| Chemical compound, drug | Phenformin hydrochloride | MilliporeSigma | Cat#PHR1573 | N/A |
| Commercial assay or kit | Quantitect Reverse Transcription Kit | QIAGEN | Cat#205314 | N/A |
| Commercial assay or kit | Quantitect SYBR Green PCR Reagent | QIAGEN | Cat#204145 | N/A |
| Chemical compound, drug | Levamisole | MilliporeSigma | Cat#L9756 | N/A |
| Software, algorithm | OASIS2 | Structural Bioinformatics Laboratory, POSTECH | https://sbi.postech.ac.kr/oasis2/surv/ | N/A |
| Software, algorithm | MetaMorph | Molecular Devices | https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy | N/A |
| Software, algorithm | Xcalibur (v4.1.31.9) | Thermo Fisher Scientific | Cat#OPTON-30965 | N/A |
| Software, algorithm | QualBrowser (v4.1.31.9) | Thermo Fisher Scientific | Cat#XCALI-97617 | N/A |
| Software, algorithm | MZmine (v2.36) | Open Source | RRID: SCR_012040, http://mzmine.github.io |
N/A |
| Software, algorithm | MetaboAnalyst (v5.0) | N/A | https://www.metaboanalyst.ca | N/A |
| Software, algorithm | CellProfiler (v4.2.1) | Broad Institute | https://cellprofiler.org | N/A |
| Software, algorithm | Prism (v9.0) | GraphPad by Dotmatics | https://www.graphpad.com/ | N/A |
| Software, algorithm | Fiji/ImageJ2 (v2.13.1) | NIH | https://imagej.net/software/fiji/ | N/A |