FIG. 2.

HSV-1-induced expression of β-galactosidase in Vero cells transformed with a Sindbis virus replicon cDNA under control of the ICP8 promoter. Several Vero cell clones transformed with pICP8SINrep/LacZ were either mock infected or infected with wild-type HSV-1 (MOI = 10) in the presence of acyclovir in the wells of a 6-well dish. At 24 h postinfection, 2 ml of lysis buffer was added and 10 μl of the extracts was assayed for β-galactosidase activity as described in Materials and Methods. V2-33 and V2-34 were independently isolated transformed clones; V2-34-8 and V2-34-17 were subclones from the original cloned V2-34 population. The results shown are the means of results from duplicate samples.