FIG. 6.

Induction of the Sindbis virus replicon in V3-45N cells by HSV-1 mutants. V3-45N cells were infected with wild-type HSV-1 (KOS), dlx3.1 or d120 (MOI = 5). Wild-type HSV-1 (KOS) and dlx3.1-infected cells were treated with acyclovir (50 μM). At 24 h after infection, the medium (2 ml) were collected and cell extracts were prepared. A sample of the medium (200 μl) was inoculated onto BHK cells in the wells of a 24-well dish. After 18 h, cell extracts were prepared with 200 μl of lysis buffer and β-Galactosidase activity was assayed as described in Materials and Methods. (A) β-Galactosidase activity of V3-45N cell extracts. (B) Titers of SINrep/LacZ in media from V3-45N cells after infection with wild-type HSV-1 (KOS), infection with mutant viruses (d120 and dlx3.1), or mock infection. The titer of SINrep/LacZ, expressed as blue-forming units (bfu), was determined from a standard curve (see inset) generated as described in Materials and Methods.