FIG. 8.
HCMV induction of β-galactosidase activity in mink lung cells transformed with pUL45SINrep/LacZ. Isolation of MLUL45SINrep/LacZ cells and MLUL45/LacZ cells is described in Materials and Methods. Each cell line was plated in the wells of a 24-well dish. The cells were infected with 50 μl of HCMV (AD169 strain; titer of 106 infectious focus units/ml) in the presence of 0.1× TurboTreat (Diagnostic Hybrids, Inc.). At 48 h after infection, the cells were treated with 200 μl of lysis buffer and 50 μl of extract was assayed for β-galactosidase activity as described in Materials and Methods. Results shown are the means of results from triplicate samples.