TABLE 1.
TCA-precipitable 35S radioactivity and inhibition of protein synthesis
| Minutes after downshift | Mean (SD) cpm of 35S label
|
% Inhibition of protein synthesisa
|
|||
|---|---|---|---|---|---|
| Untreated | Cyclo-heximide added | ATP depleted | Cyclo-heximide added | ATP depleted | |
| −30b | 250 (6) | ||||
| 40 | 11,989 (432) | 768 (22) | 2,747 (279) | 93.6 | 77.1 |
| 150 | 19,975 (863) | 1,119 (102) | 3,595 (276) | 94.4 | 82 |
a
Calculated by comparing mean incorporated counts per minute with untreated control.
b
Sample collected 30 min before downshift, at time of addition of radiolabel.