Figure 1.
iNPC-GDNF and fNPC-GDNF profiles
(A) Protocol to generate and expand iNPC-GDNFs.
(B) Unbiased UMAP clustering of cells from fNPC-GDNF and iNPC-GDNF neurospheres split into 11 defined clusters.
(C) Clustering split by cell type shows that iNPC-GDNFs clustered similarly to fNPC-GDNFs in many but not all cases.
(D and E) Feature plots show expression of (D) cortical neural progenitor markers and (E) astrocyte markers.
(F) Immunocytochemistry on fNPC-GDNFs and iNPC-GDNFs differentiated for 7 days in culture shows production of S100β and GFAP, with DAPI (blue). Scale bar represents 75 μm.