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. 2023 Apr 20;18(8):1629–1642. doi: 10.1016/j.stemcr.2023.03.016

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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iNPC-GDNF spinal cord long-term grafts

(A) Schematic of experiment. Rats (n = 10) were unilaterally transplanted with 100,000 iNPC-GDNFs in three sites 2 mm apart in the lumbar spinal cord, with euthanasia at 3 months (n = 1) and 9 months (n = 9) post-transplantation.

(B and C) Grafts at 9 months were stained for (B) GDNF and (C) pan-GFAP and SC121 with DAPI (blue).

(D and E) SC121 and IBA1 staining (D) and quantification of IBA1+ cells with a paired t test (E) (average per animal, minimum of four sections analyzed per animal). ns: not significant. Scale bars represent (B) 75 μm, (C) 20 μm, and (D) 50 μm.

See also Figure S4.