Figure 4.

Grafted human lt-NES cell-derived oligodendrocytes myelinate axons in the rat injured cortex

(A and B) Light micrographs of a toluidine blue-stained plastic-embedded section from the cortex of stroke-subjected rats contralateral (A) and ipsilateral (B) to the lesion. GFP/DAB-positive lt-NES cell-derived cells were visualized in the peri-infarct area (ipsilateral cortex), corpus callosum, and contralateral cortex. Arrows highlight GFP/DAB-positive cells (brown color), and arrowheads indicate GFP/DAB-positive processes (brown color).

(C) Representative iEM image showing that, at 6 months after transplantation, GFP/DAB-positive cells in the corpus callosum exhibit the morphology of mature myelinating oligodendrocytes (for better visualization in the EM images, GFP/DAB-positive oligodendrocyte cytoplasm and processes are colored green and the nucleus is yellow). The host axon myelinated by the GFP/DAB-positive oligodendrocyte is colored blue. Red arrowheads indicate areas of GFP/DAB-positive myelin.

(D) Electron micrographs of GFP⁺ immunogold particles (green circles) associated with compact myelin sheaths. CC, corpus callosum; m, mitochondrion. Scale bars, 50 μm (A and B), 2 μm (C), and 0.1 μm (D).