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cDNA synthesis reaction components.
| Reaction component | Amount |
|---|---|
| RNA | 1 µg |
| Random Hexamers | 2.5 µL (from 100 µM stock) |
| 5X Enzyme Buffer | 5 µL |
| dNTP | 1.7 µL (from 40 mM stock) |
| AMV-RT (20U) | 2 µL |
| RNase free water | q.s. (quantity sufficient) |
| Total volume | 25 µL |
