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. 2023 Aug 22;13:13663. doi: 10.1038/s41598-023-40808-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Improved growth-based sensor in NLRs. Panel a: Brightfield and b: green fluorescence microscopy pictures of NLRs harboring the improved growth-bases biosensor (average occupation of 200 cells/NLR). The NLRs were incubated for 2 days at 37 °C in M9 medium supplemented with (1) 0, (2) 2, (3) 4, (4) 8, (5) 16 and (6) 32 mM GABA. Exposure time for (2–6) was 100 ms and for (1) 1 s. Panel c: Response curve of the growth-based biosensor in NLRs upon addition of different concentrations of GABA. The values plotted represent the mean of the integrated green fluorescence measured with flow cytometry, while the error bars represent the standard deviation of the 1667, 1887, 1024, 993, 1360, 1587 events recorded for each concentration (0–32 mM GABA).