Table 1. Genome data for Bombus pascuorum, iyBomPasc1.1.
| Project accession data | ||
|---|---|---|
| Assembly identifier | iyBomPasc1.1 | |
| Species | Bombus pascuorum | |
| Specimen | iyBomPasc1 | |
| NCBI taxonomy ID | 65598 | |
| BioProject | PRJEB43540 | |
| BioSample ID | SAMEA7520484 | |
| Isolate information | iyBomPasc1, male, head and thorax (DNA sequencing and Hi-C
scaffolding) iyBomPasc2, thorax (RNA sequencing) |
|
| Assembly metrics * | Benchmark | |
| Consensus quality (QV) | 51.4 | ≥ 50 |
| k-mer completeness | 99.98% | ≥ 95% |
| BUSCO ** | C:97.8%[S:97.5%,D:0.3%],
F:0.4%,M:1.8%,n:5,991 |
C ≥ 95% |
| Percentage of assembly mapped to chromosomes | 87.82% | ≥ 95% |
| Sex chromosomes | Not applicable | localised homologous pairs |
| Organelles | Mitochondrial genome assembled | complete single alleles |
| Raw data accessions | ||
| PacificBiosciences SEQUEL II | ERR6548408 | |
| 10X Genomics Illumina | ERR6054545–ERR6054548 | |
| Hi-C Illumina | ERR6054549, ERR6054550 | |
| PolyA RNA-Seq Illumina | ERR6054551, ERR6286716 | |
| Genome assembly | ||
| Assembly accession | GCA_905332965.1 | |
| Accession of alternate haplotype | GCA_905332995.1 | |
| Span (Mb) | 307.5 | |
| Number of contigs | 120 | |
| Contig N50 length (Mb) | 9.0 | |
| Number of scaffolds | 81 | |
| Scaffold N50 length (Mb) | 17.6 | |
| Longest scaffold (Mb) | 35.1 | |
| Genome annotation | ||
| Number of protein-coding genes | 12,999 | |
| Number of non-protein coding genes | 5,442 | |
| Average length of coding sequence (bp) | 12,765.73 | |
| Average number of exons per transcript | 6.13 | |
| Average number of introns per transcript | 5.13 | |
*Assembly metric benchmarks are adapted from column VGP-2020 of “Table 1: Proposed standards and metrics for defining genome assembly quality” from ( Rhie et al., 2021).
**BUSCO scores based on the hymenoptera_odb10 BUSCO set using v5.3.2. C = complete [S = single copy, D = duplicated], F = fragmented, M = missing, n = number of orthologues in comparison. A full set of BUSCO scores is available at https://blobtoolkit.genomehubs.org/view/iyBomPasc1.1/dataset/CAJOSQ01/busco.