Table 2.
Summary of methods used to isolate the CNS-derived EVs currently.
| Type of EVs | Marker antibody target | Target expression in known brain cell type | Target expression in tissue | EV isolation methods | No. of paper |
|---|---|---|---|---|---|
| NDEs | L1CAM | Neuron | Brain, intestine, monocyte, testis | Precipitation + immunoaffinity | 44 |
| Immunoaffinity | 4 | ||||
| Centrifugation + immunoaffinity | 3 | ||||
| Size exclusion chromatography + immunoaffinity | 1 | ||||
| NCAM | Neuron, oligodendrocyte | Brain, heart muscle | Precipitation + immunoaffinity | 3 | |
| SNAP25 | Neuron | Brain, retina | Immunoaffinity | 1 | |
| ADEs | GLAST | Astrocyte | Brain, heart, ovary, placenta | Precipitation + immunoaffinity | 8 |
| Immunoaffinity | 1 | ||||
| AQP4/GFAP | Astrocyte | Brain, lung/brain, kidney | Centrifugation + flow cytometry | 1 | |
| ODEs | OMG | Oligodendrocyte, Astrocyte | Brain, choroid plexus | Immunoaffinity | 1 |
| CNP | Oligodendrocyte | Brain, retina | Immunoaffinity | 1 | |
| MOG | Oligodendrocyte | Brain, spinal cord | Precipitation + immunoaffinity | 1 |