Table 1.
Evaluation of affinity of anti-thrombin aptamers to thrombin by SPR
| Aptamer | Human thrombin |
Mouse thrombin |
||||
|---|---|---|---|---|---|---|
| ka (1/Ms) | kd (1/s) | KD (nM) | ka (1/Ms) | kd (1/s) | KD (nM) | |
| M08s | 7.04 × 105 | 3.33 × 10−2 | 47.2 | 3.56 × 105 | 1.76 × 10−1 | 495 |
| TBA29 | 1.34 × 105 | 4.94 × 10−3 | 36.9 | 2.76 × 105 | 6.07 × 10−3 | 22.0 |
| Lin(08-08) | 1.63 × 106 | 6.94 × 10−4 | 0.4 | 6.41 × 105 | 4.30 × 10−3 | 6.7 |
| Pse(08-08) | 1.19 × 106 | 5.10 × 10−4 | 0.4 | 7.35 × 105 | 3.06 × 10−3 | 4.2 |
| Pse(08–29) | 1.33 × 106 | 3.47 × 10−3 | 2.6 | 6.72 × 105 | 4.26 × 10−3 | 6.3 |
Human or mouse thrombin was immobilized to the CM5 chip with a low RU of 500. The KD of Pse(08–29) was calculated as 1:1 binding by approximating that M08s and TBA29 possess the same KD as the homodimer.