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. 2023 Aug 9;10:1220198. doi: 10.3389/fvets.2023.1220198

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Copyright © 2023 Jadhav, Gaonkar, Joshi and Rathi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Protein digestibility (%) and (B) Degree of hydrolysis (%) after gastric and gastro-intestinal digestion of the canine food in absence and presence of enzyme blend. (C) Increase in amino acid concentration (%) after gastro-intestinal (GI) digestion (digestion time—0.5, 1, and 2 h) of the canine food in presence of enzyme blend. (D) Increase in AUC (%) after gastric (G) and gastrointestinal (GI) digestion (digestion time—0.5, 1, 2, and 3 h) of the canine food in presence of enzyme blend. Values are represented as mean ± standard deviation. Two-way ANOVA with Tukey’s multiple-comparison test was used to determine the p value. ^*, ^**, and ^*** represent significant difference at p ≤ 0.05, p ≤ 0.005, and p ≤ 0.001, respectively.