Figure 10.

The working hypothesis for the mechanism of miR-10a-5p overexpression in OCCC G₀ or G₁/S Checkpoint. (A) Simplified representation of cell cycle progression function in non-cancerous cells, whereby CDK6 phosphorylates Rb freeing E2F for DNA replication in the S phase. (B) Infographic representation of cell cycle progression in non-malignant cells treated with platinum and/or taxane-containing agents. Cells will sustain DNA and/or microtubule damage resulting in no continued progression through the cell cycle and subsequent cell death. (C) Represents the working hypothesis for cell cycle progression in miR-10a-5p overexpressing OCCC cells. MiR-10a-5p downregulates CDK6 and other important regulators of the cell cycle slowing or halting phosphorylation of Rb leading to inactive or prolonged inactivation of E2F and transition to S Phase and DNA replication. Cells slowed in G₁ or senescing in G₀ miss the critical chemotherapeutic effects in the S and M phases (red “X”s).