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. 2023 Aug 3;36(8):1398–1408. doi: 10.1021/acs.chemrestox.3c00140

Figure 1.

Figure 1

Dose–response curves of MPO inhibition for acetaminophen, AMBB, and AAPBA determined by the taurine N-chloramine/TMB assay (A) and NBD-TM oxidation (B). Mixtures contained taurine (20 mM) or NBD-TM (20 μM), MPO (0.1 nM, 5 nM HOCl/s), H2O2 (10 μM), NaCl (0.1 M), phosphate buffer (20 or 50 mM, pH 7.4), 3% (v/v) methanol, and the appropriate compound AM (0.01 μM–6 mM), AMBB (0.01–300 μM), or AAPBA (0.01 μM–6 mM). The experimental points were read for the incubation time of 5 min. Each panel is a representative result of three independent experiments and points represent means ± S.D.