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. 2023 Aug 3;36(8):1398–1408. doi: 10.1021/acs.chemrestox.3c00140

Figure 2.

Figure 2

UPLC-MS analyses of AMBB and HOCl reaction products. (A) UPLC traces of samples containing 50 μM AMBB, various concentration of HOCl (0–50 μM), and 20 mM phosphate buffer (pH 7.4). Samples were analyzed immediately (<2 min) after mixing. (B) Spontaneous conversion of AMB-OH to acetaminophen (AM). Incubation mixture contained 50 μM AMBB, 50 μM HOCl, and 20 mM phosphate buffer. UPLC traces were extracted at 250 ± 1.2 nm. m/z values for [M + H]+ ions were obtained using a TOF-MS detector (see Supporting Information). Each panel is a representative result of three independent experiments.