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Kinetics of the conversion of AMB-OH into acetaminophen (AM). AMB-OH was produced in situ by mixing 50 μM AMBB with 50 μM NaOCl in 20 mM phosphate buffer, pH 7.4. (A) UPLC peak areas of AMB-OH (open squares) and AM (open circles) as a function of incubation time. UPLC peak areas were integrated for chromatograms extracted at 250 ± 1.2 nm. (B) Scheme of the quinone methide moiety elimination by AMB-OH to form AM.
