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. 2023 Aug 3;36(8):1398–1408. doi: 10.1021/acs.chemrestox.3c00140

Figure 7.

Figure 7

Effect of uric acid and HSA on the extent of oxidation of AMBB by HOCl and ONOO–. (A) UPLC peak areas of AMB-OH. Incubation mixtures contained 100 μM AMBB, 50 μM HOCl, or ONOO–, 0–400 μM uric acid, 20 mM phosphate buffer (pH 7.4), and 5% (v/v) ACN. Samples were analyzed immediately after mixing. (B) UPLC peak areas of acetaminophen. Incubation mixtures contained 100 μM AMBB, 50 μM HOCl, or ONOO–, 0–700 μM HSA, 20 mM phosphate buffer (pH 7.4), and 5% (v/v) ACN. Samples were analyzed 24 h after mixing. UPLC peak areas were integrated for chromatograms extracted at 250 ± 5 nm. Points represent means ± S.D. for at least two independent measurements.