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. Author manuscript; available in PMC: 2024 Apr 27.
Published in final edited form as: Methods Enzymol. 2023 Apr 27;685:171–198. doi: 10.1016/bs.mie.2023.03.013

Figure 1. The catalytic cycle of a protein kinase.

Figure 1.

ATP binds to the active site of the kinase, which consists of an N-terminal and C-terminal lobe. The γ-phosphate of ATP is hydrolyzed and transferred to the hydroxyl oxygen of a protein target residue side chain (Ser, Thr or Tyr) and concomitant production of ADP.