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. 2023 Aug 22;11(8):e006944. doi: 10.1136/jitc-2023-006944

Figure 5.

Figure 5

Mut1 and Mut5,6 are the only protective epitopes in the VH of M315. (A) Overview of mutant VH used in scFv constructs used for immunization. All scFv constructs included VL 315. Germline is shown in black, M315 in orange. Below, constructs are abbreviated ΔMut1 etc. (B, C) BALB/c mice were immunized with 50 µg DNA or NaCl i.m./EP, and challenged 14 days later intravenous with MOPC315.BM.Luc.IgAλ2 (2×105 cells). Shown are bioluminescence measurements (mean±SEM) and representative ventral images on day 35 (left), M315 levels in sera at endpoint (mean±SEM, middle) and survival (right). n=5–8 mice/group. Statistics: Two-way ANOVA with Tukey’s post-test (bioluminescence), One-way ANOVA with Tukey’s post-test (M315 measurements) and Mantel Cox log-rank test (survival curves) compared with NaCl group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ANOVA, analysis of variance; ND, not detected; ns, not significant; scFv, single-chain fragment variable.