TABLE 3.

Amino acid and nucleotide sequences of the TCR β-chains of peptide 69-specific CTL clones from donor 011

Date of blood sample	Formal clone or CTL culture	% Specific lysis		TCR β-chain sequence
		With peptide	Control	Vβ	CDR3	Jβ	Cβ
11/96a	Clone 2	89	4	Vβ17.1	C   A   S     M   G   N   S   A   G   A   N   V   L   T     F   G	Jβ2.6	Cβ2
					tgt gcc agt   atg ggt aat tca gct ggg gcc aac gtc ctg act   ttc ggg
5/97b	Culture 2	56	0	Vβ17.1	C   A   S     M   G   N   S   A   G   A   N   V   L   T     F   G	Jβ2.6	Cβ2
					tgt gcc agt   atg ggt aat tca gct ggg gcc aac gtc ctg act   ttc ggg
	Culture 3	21	0	Vβ17.1		Jβ2.6	Cβ2
	Culture 4	22	0	Vβ17.1		Jβ2.6	Cβ2
	Culture 6	28	0	Vβ17.1		Jβ2.6	Cβ2
	Culture 8	39	0	Vβ17.1		Jβ2.6	Cβ2
11/96c	Clone 1	88	3	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
					tgt gcc agc   agt tca gct aac tat ggc tac acc ttc ggt
	Clone 3	87	8	Vβ8		Jβ1.2	Cβ1
5/97d	Culture 1	54	2	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
					tgt gcc agc   agt tca gct aac tat ggc tac acc ttc ggt
	Culture 7	27	0	Vβ8	C   A   S     S   S   V   S   E   A   F   F   G	Jβ1.1	Cβ1
					tgt gcc agc   agt tct gtc tct gaa gct ttc ttt gga
	Culture 9	39	0	Vβ8	C   A   S     S   S   V   S   E   A   F   F   G	Jβ1.1	Cβ1
					tgt gcc agc   agt tct gtc tct gaa gct ttc ttt gga
	Culture 10	34	0	Vβ8	C   A   S     S   S   V   N   E   Q   F   F   G	Jβ2.1	Cβ2
					tgt gcc agc   agt tca gtt aat gag cag ttc ttc ggg
4/98e	Culture 3	53	0	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
	Culture 4	75	0	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
	Culture 7	41	1	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
	Culture 8	76	2	Vβ8	C   A   S     S   S   A   N   Y   G   Y   T   F   G	Jβ1.2	Cβ1
	Culture 2	62	0	Vβ8	C   A   S     S   S   V   S   E   A   F   F   G	Jβ1.1	Cβ1
	Culture 5	41	1	Vβ8	C   A   S     S   S   V   S   E   A   F   F   G	Jβ1.1	Cβ1
	Culture 6	72	0	Vβ8	C   A   S     S   S   V   S   E   A   F   F   G	Jβ1.1	Cβ1
	Culture 1	55	1	Vβ8	C   A   S     S   S   V   N   E   Q   F   F   G	Jβ2.1	Cβ2

^aSequence of one Vβ17⁺ formal clone. The CDR3 region is as defined by Kabat and Wu (21) as starting at position 95 of Vβ and finishing at the phenylalanine of the motif FGXG within Jβ. 

^bSequences of five independent Vβ17⁺ CTL cultures. (All five sequences were identical.) 

^cSequences of two independent Vβ8⁺ formal clones. (The two sequences were identical.) 

^dSequences of four independent Vβ8⁺ CTL cultures. 

^eSequences of eight independent Vβ8⁺ CTL cultures.