TABLE 6.
Clonal focusing of CTL specific to HLA-B7-restricted pp65 peptide 56
| Donora | Date of blood sample | Formal clone or CTL culture | TCR β-chain sequence
|
TCR α-chain sequence
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Vβ | CDR3 | Jβ | Cβ | Vα | CDR3 | Jα | |||
| 002 | 5/98 | Clone A | Vβ6.4 | C A S S S H D T G G Y N S P L H F G | Jβ1.6 | Cβ1 | |||
| Culture 5 | Vβ6.4 | C A S S S H D T G G Y N S P L H F G | Jβ1.6 | Cβ1 | |||||
| Clone B | Vβ6.4 | C A S S F R D Y G N Y E Q Y F G | Jβ2.7 | Cβ2 | |||||
| Clone C | Vβ6.4 | C A S S F R D Y G N Y E Q Y F G | Jβ2.7 | Cβ2 | |||||
| Culture 7 | Vβ6.4 | C A S S F R D Y G N Y E Q Y F G | Jβ2.7 | Cβ2 | |||||
| 009 | 10/97 | Clone B56 | Vβ14.1 | C A S S I G P A L N T E A F F G | Jβ1.1 | Cβ1 | |||
| Clone C56 | Vβ14.1 | C A S S I G P A L N T E A F F G | Jβ1.1 | Cβ1 | |||||
| 2/98 | Clone 1a | Vβ14.1 | C A S S I G P A L N T E A F F G | Jβ1.1 | Cβ1 | ||||
| Clone 1b | Vβ14.1 | C A S S I G P A L N T E A F F G | Jβ1.1 | Cβ1 | |||||
| Clone 2a | Vβ14.1 | C A S S I G P A L N T E A F F G | Jβ1.1 | Cβ1 | |||||
| 015 | 1/97 | Clone 5 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | Vα6.1 | C A M R E G M D S S Y K L I F G | Jα31 |
| Clone 7 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | Vα6.1 | C A M R E G M D S S Y K L I F G | Jα31 | ||
| Bulk CTL line | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | Vα6.1 | C A M R E G M D S S Y K L I F G | Jα31 | ||
| 7/97 | Clone 3 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | ||||
| 1/98 | Clone 5 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | ||||
| 3/98 | Clone 2 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | ||||
| Clone 5 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | |||||
| Clone 6 | Vβ6.4 | C A S S L H D R G S R T E A F F G | Jβ1.1 | Cβ1 | |||||
| Clone 3 | Vβ14.1 | C A S K V G A G G L Y E Q Y F G | Jβ2.7 | Cβ2 | |||||
| 016 | 7/97 | Clone 8 | Vβ6.4 | C A S S L I G V S S Y N E Q F F G | Jβ2.1 | Cβ2 | Vα3.1 | C A T V L R M D S S Y K L I F G | Jα21 |
| 9/97 | Clone 4 | Vβ6.4 | C A S S L I G V S S Y N E Q F F G | Jβ2.1 | Cβ2 | Vα3.1 | C A T V L R M D S S Y K L I F G | Jα31 | |
| Bulk CTL line | Vβ6.4 | C A S S L I G V S S Y N E Q F F Q | Jβ2.1 | Cβ2 | Vα3.1 | C A T V L R M D S S Y K L I F G | Jα31 | ||
| H0018 | 1/98 | Clone 1B | Vβ6.4 | C A S S S H D R Q G A S S P L H F G | Jβ1.6 | Cβ1 | |||
a
TCR α- and β-chain amino acid sequences are shown from CTL clones derived from five donors. These TCRs exclusively use Vβ6.4 or Vβ14. CTL from donors 015 and 016 also both use Jα31.