Fig. 3.

The repressive effect of TOF on influenza A/PR/8/34-GFP viral RNA and protein expression. (A) HA, M2, and NP protein expression. TOF (100 μg/ml) or mock was co-treated to RAW 264.7 cells. At 24 h post-infection, the cells were fixed with paraformaldehyde, stained with antibodies against (HA, M2, and NP) (Red), and Hoechst 33,342 for nuclei (Blue). The viral protein and nuclei were observed and captured under a fluorescent microscope. (B) Viral RNA level in RAW 264.7 cells with different dosages of TOF. At 18 h post-infection, total RNA was isolated from cells. After synthesizing cDNA, q-PCR was performed using viral-specific primers. M; Mock group, V; Virus-only group, V/TOF; Virus and TOF cotreated group. The data represent the mean ± SD based on three independent experiments. Statistical significance was assessed via an unpaired Student T-test. ***P<0.001 compared with the virus-infected group.