Fig. 9.

Anti-influenza viral effect of five constituents in TOF. Each compound (50 μM) or TOF (100 μg/ml) was mixed with PR8-GFP IAV (10 MOI). The cytotoxicity of each compound was determined using a CCK-8 assay. Each compound was not toxic at 50 μM (Sup. Fig. 1). The mixtures were added to RAW 264.7 cells and incubated for GFP expression. Fluorescence and brightfield images were captured (A) and GFP expression was analyzed by FACS (B, C). ^⁎⁎p< 0.01 and ^⁎⁎⁎p< 0.001 compared with the virus-infected group. M; Mock group.