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. 2023 Mar 3;114(5):513–520. doi: 10.1093/jhered/esad016

Table 2.

Assembly metrics comparing previous Eublepharis macularius reference genome (Xiong et al. 2016) to MPM_Emac_v1.0 reference assembly.

Metric Xiong et al. 2016 MPM_Emac_v.1.0
Contig N50 (bp)* 20,426 80,105,973
Contig L50* 27,671 9
Scaffold N50 (bp) 663,762 145,573,841
Scaffold L50 796 6
Repeat content % 42.1% 48.0%
GC content % 43.6% 44.1%
Total scaffolds 206,349 75
Genome Size (bp)* 1,981,651,937 2,237,374,058
BUSCO Sauropsida (Genome) C:93.4%[S:91.4%,D:2.0%],F:2.0%,M:4.6%,n:7480 C:95.1%[S:93.0%,D:2.1%],F:0.9%,M:4.0%,n:7480
BUSCO Sauropsida (Annotation) C:90.6%[S:88.7%,D:1.9%],F:3.2%,M:6.2%,n:7480 C:89.3%[S:87.6%,D:1.7%],F:3.6%,M:7.1%,n:7480
BUSCO CVG (Genome) C:96.6%[S:95.7%,D:0.9%],F:2.1%,M:1.3%,n:233 C:99.2%[S:97.9%,D:1.3%],F:0.9%,M:0.1%,n:233

BUSCO scores abbreviated as follows: C = complete, S = complete single copy, D = complete multi-copy, F = fragmented, M = missing.

*Missing data not included in calculation.