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. 2023 Aug 9;14:1164080. doi: 10.3389/fimmu.2023.1164080

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Copyright © 2023 Zupancic, Smith, Trzeciakiewicz, Skinner, Ferris, Makowski, Lucas, McArthur, Kane, Paulson and Tessier

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunodot analysis of tau conformational nanobodies using mouse brain samples. Immunodot blotting analysis of the selected nanobody-Fc fusion proteins (WA2.22 and WA2.21) was evaluated for both wild-type and transgenic P301S mouse brain homogenates. For comparison, a conformational tau antibody (zagotenemab) and a sequence-specific tau antibody (Tau-5) were also analyzed. Immunoblots were imaged at both short (left) and long (right) exposure times. Ponceau stain was used as a loading control. The experiment was repeated twice, and a representative image is shown.