Figure 4.

Immunofluorescence analysis of a tau conformational nanobody using mouse brain samples. Immunofluorescent staining of fixed brain sections from wild-type and transgenic P301S mice was performed using WA2.22 (purple; Fc fusion protein). Tissue sections were co-stained with a phospho-tau antibody (AT8, green) and DAPI (blue). WA2.22 signal was detected using Alexa Fluor 647, and AT8 signal was detected using Alexa Fluor 488. The scale bars in the images represent approximately 50 µm, and the scale bars in the insets represent approximately 20 µm.