Figure 5.

Immunofluorescence analysis of a tau conformational nanobody using human brain samples. Immunofluorescent staining of fixed sections from human brains without cognitive impairment (control), Alzheimer’s disease (AD), and progressive supranuclear palsy (PSP) was performed using WA2.22 (purple; Fc fusion protein). Tissue sections were co-stained with a phospho-tau antibody (AT8, green) and DAPI (blue). WA2.22 signal was detected using Alexa Fluor 647, and AT8 signal was detected using Alexa Fluor 488. The scale bars in the images represent approximately 50 µm.